Macrowine scientific programme


	SCIENTIFIC PROGRAMME Wednesday, June 16 8.30 - 9.00 Registration of partecipants 9.00 – 9.20 Opening SESSION I CHEMISTRY, FUNCTIONALITY AND ANALYSIS OF GRAPE AND WINE MACROMOLECULES AND SECONDARY METABOLITES Chaiperson: Véronique Cheynier 9.20 - 10.00 Keynote CHEMISTRY AND ANALYSIS OF KEY VOLATILE COMPOUNDS OF WINE AND THEIR PRECURSORS IN GRAPE Philippe Darriet, University of Bordeaux 2,Villenave d'Ornon, France 10.00 – 10.40 Invited: Dalmasso Lecture Wine oxidation: recent revelations Andrew Waterhouse, University of California, Davis, USA 10.40 – 11.00 Characterization and formation kinetic study of phenolic compounds-volatile thiol adducts by chemical and enzymatic oxidation Maria Nikolantonaki, Institut des Sciences de la Vigne et du Vin,Villenave d'Ornon, France 11.00 - 11.30 Coffee break and poster viewing 11.30 – 11.50 Preliminary studies on polyphenol assessment by fourier transform-near infrared spectroscopy (FT-NIR) in berries Chiara Pagliarani, University of Torino, Torino, Italy 11.50 – 12.10 A metabolomic view of wine micro-oxygenation Fulvio Mattivi, Fondazione Edmind Mach, San Michele All'Adige, Trento, Italy 12.10 – 12.30 Effects of ionic strength and sulfate upon thermal aggregation of grape chitinases and thaumatin-like proteins in model system Matteo Marangon, The Australian Wine Research Institute, Glen Osmond, Australia 12.30 - 12.50 Progress in precision viticulture for the assessment of grape anthocyanins at harvest in australian vineyards Marine Le Moigne, Centre Universitaire Orsay, Orsay Cedex, France 13.00 - 14.00 Lunch SESSION II BERRY DEVELOPMENT AND BIOSYNTHESIS OF GRAPE SECONDARY METABOLITES Chairman: Serge Delrot 14.00 - 14.40 Keynote REGULATION OF FLAVONOID BIOSYNTHESIS DURING GRAPE BERRY DEVELOPMENT Jochen Bogs, University of Heidelberg, Heidelberg, Germany 14.40 - 15.00 Grapevine anthocyanin transport involves in vivo vesicle-mediated trafficking and the contribution of anthomate transporters and GST Agnes Ageorges, INRA - UMR SPO, Montpellier, France 15.00 - 15.20 Cellular localization of leucoanthocyanidin reductase and anthocyanidin reductase in grape skins of vitis vinifera L. cv. Cabernet-Sauvignon during development Soizic Lacampagne, Institut des Sciences de la Vigne et du Vin, Villenave d'Ornon, France 15.20 - 15.40 Cloning and characterization of putative anthocyanin acyltransferases from grape and petunia S. Provenzano, University of Torino, Torino, Italy 15.40 - 16.10 Coffee break and poster viewing 16.10 - 16.30 New mybs through the glass: a deeper look into the regulatory network of flavonoid synthesis in wine grapes J. Tomas Matus, Center for Research in Agricultural Genomics, Barcelona, Spain 16.30 - 16.50 Study of genetic determinants of flavonol content in grape berry skin Giulia Malacarne, Fondazione Edmund Mach, San Michele All'Adige,Trento, Italy 16.50 - 17.10 Are VvMYB5A and VvMYB5B regulators of grape flavonoid pathway? Giambattista Tornielli, University of Verona, Verona, Italy 17.10 - 17.30 Investigation of VvDXS function and its effects on Muscat flavor levels Juri Battilana, IASMA Research and Innnovation Centre, San Michele All'Adige,Trento, Italy 17.30 - 17.50 Microclimate influence on gene expression and metabolite accumulation in grape berries Christian Kappel, University of Bordeaux, Bordeaux, France 18.00 - 20.00 Welcome cocktail Thursday, June 17 SESSION III MOLECULAR EFFECTS OF STRESS AND VINEYARD MANAGEMENT Chairman: Vittorino Novello 9.00 - 9.40 Keynote POSTHARVEST ELICITORS AND METABOLIC CHANGES IN WINE GRAPE BERRIES Piero Tonutti, Scuola Superiore Sant’Anna, Pisa, Italy 9.40 - 10.00 In vivo cellular localisation of stilbene fluorescence induced by Plasmopara viticola in grapevine leaves Sebastien Bellow, Université Paris-Sud 11, Orsay, France 10.00 - 10.30 Biological role of resveratrol in plants Philippe Jeandet, University of Reims, Reims, France 10.30 - 10.50 Effect of Botrytis Cinerea and Esca on berries and wines phenolic composition Isabelle Ky, Institut des Sciences de la Vigne et du Vin, Villenave d'Ornon, France 10.50 - 11.10 Study of the early synthesis of resveratrol and its localization in grapevine cell cultures David Donnez, UFR Reims, URVVC-SE, Reims, France 11.10 - 11.30 Coffee break and poster viewing 11.30 - 11.50 Relationship between physical and mechanical berries properties and phenol extractability of different varieties of Vitis Vinifera L. grapes René Siret, Ecole Supérieure d’Agriculture, Angers Cedex, France 11.50 - 12.10 Effects of cluster thinning on berry ripening in cv. Sangiovese: agronomical, biochemical and molecular approach Chiara Pastore, University of Bologna, Bologna, Italy 12.10 - 12.30 Evaluation of winegrape anthocyanins in the vineyard using a portable fluorimetric sensor: seasonal and water regime effects Giovanni Agati, IFAC-CNR, Sesto F.no, Firenze, Italy 12.30 - 13.30 Lunch 13.30 - 23.00 Visit to the Castle of Cisterna d'Asti, to the Tenimenti di Fontanafredda Winery (Serralunga d'Alba) and social dinner at the Villa Contessa Rosa Resturant Friday, June 18 SESSION IV RELATIONS BETWEEN SECONDARY METABOLITES AND WINE SENSORY PROPERTIES Chiarman: Vincenzo Gerbi 9.00 - 9.40 Keynote CURRENT STATUS IN POLYSACCHARIDE AND OLIGOSACCHARIDE COMPOSITION OF WINES Thierry Doco, INRA Montpellier, France 9.40 - 10.00 Toward a model of grape proanthocyanidin extraction during vinification Keren Bindon, Australian Wine Research Institute, Adelaide, Australia 10.00 - 10.20 Ultra high pressure liquid chromatography for stilbenes separation and their determination in Burgundy red wines Lemia Boutegrabet, Burgundy University, Dijon, France 10.20 - 10.40 Stilbene-enriched red quality wine upon UVC postharvest treatment Emma Cantos-Villar, IFAPA, Jerez de la Frontera, Cadiz, Spain 10.40 - 11.00 Evolution of biogenic amines content in wine during sample conservation - method optimisation for analysis of biogenic amines Benoit Bach, Inter Rhone, Orange, Cadiz, France 11.10 - 11.30 Coffee break and poster viewing 11.30 -11.50 Effect of winemaking techniques on the polysaccharide and oligosaccharide composition of Cabernet Sauvignon, Syrah and Monastrell red wines Rafael Apolinar-Valiente, University of Murcia, Murcia, Spain 11.50 -12.10 Phenolic composition of bordeaux grapes 2009 vintage: comparison with 2006, 2007 and 2008 vintages Bénédicte Lorrain, Institute des sciences de la Vigne et du Vin, Villenave d'Ornon, France 12.10 - 12.30 PH and ionic strength impact on protein stability in model white wines at room temperature Marie Dufrechou, INRA, Montpellier, France 12.30 - 12.50 Changes in wine secondary metabolites composition by the timing of inoculation with lactic acid bacteria: impact on wine aroma Guillaume Antalick, ISVV Faculty D'Oenologie Bordeaux, Villenave d'Ornon, France 13.00 - 14.00 Lunch SESSION V IMPACT OF MICROBIOLOGICAL AND ENOLOGICAL TECHNIQUES ON WINE MACROMOLECULES Chairperson: Elizabeth Waters 14.00 - 14.40 Keynote CHEMICAL SYSTEMS BEHIND WINE AROMA PERCEPTION: OVERVIEW, GENESIS AND EVOLUTION Ana Escudero, University of Zaragoza, Zaragoza, Spain 14.40 - 15.00 Chemical and sensory evaluation of Bordeaux wines (Cabernet-Sauvignon and Merlot) and correlation with wine age Kleopatra Chira, Institute des sciences de la Vigne et du Vin, Villenave d'Ornon, France 15.00 -15.20 Different oxygen and sulphur dioxide concentrations in Sauvignon blanc must: effect on the composition of the must and wine Wessel Du Toit, University of Stellenbosch, Cape Town, South Africa 15.20 - 15.40 Identification, quantification and organoleptic impact of 3-METHYL-2, 4-NONANEDIONE, a new volatile compound associated with the oxidation of red wines Alexandre Pons, Institute des sciences de la Vigne et du Vin, Villenave d'Ornon, France 15.40 - 16.10 Modulation of wine aroma composition and sensory properties through management of fermentation and post-fermentation enological variables Maurizio Ugliano, Australian Wine Research Institute, Glen Osmond SA, Australia 16.10 - 16.30 Coffee break and poster viewing 16.30 - 16.50 Brettanomyces Bruxellensis and off-odours: genetic and proteomic approaches to unravel the molecular mechanism of ethyl phenols production Luca Cocolin, University of Torino, Torino, Italy 16.50 - 17.10 Sensory evaluation of Sauvignon blanc grapes by a trained panel Erna Witbooi, University of Stellenbosch, Cape Town, South Africa 17.10 - 17.30 Extraction and identification of phenolic and volatile compounds in cork closures Maria A. Silva, Institut des Sciences de la Vigne et du Vin, Villenave d'Ornon, France 17.30 - 18.00 Final discussion

SCIENTIFIC PROGRAMME

Wednesday, June 16

8.30 - 9.00 Registration of partecipants

9.00 – 9.20 Opening
SESSION I
CHEMISTRY, FUNCTIONALITY AND ANALYSIS OF GRAPE AND WINE MACROMOLECULES AND SECONDARY METABOLITES
Chaiperson: Véronique Cheynier

9.20 - 10.00
Keynote
CHEMISTRY AND ANALYSIS OF KEY VOLATILE COMPOUNDS OF WINE AND THEIR PRECURSORS IN GRAPE
Philippe Darriet, University of Bordeaux 2,Villenave d'Ornon, France

10.00 – 10.40
Invited: Dalmasso Lecture
Wine oxidation: recent revelations
Andrew Waterhouse, University of California, Davis, USA

10.40 – 11.00
Characterization and formation kinetic study of phenolic compounds-volatile thiol adducts by chemical and enzymatic oxidation
Maria Nikolantonaki, Institut des Sciences de la Vigne et du Vin,Villenave d'Ornon, France

11.00 - 11.30
Coffee break and poster viewing

11.30 – 11.50
Preliminary studies on polyphenol assessment by fourier transform-near infrared spectroscopy (FT-NIR) in berries
Chiara Pagliarani, University of Torino, Torino, Italy

11.50 – 12.10
A metabolomic view of wine micro-oxygenation
Fulvio Mattivi, Fondazione Edmind Mach, San Michele All'Adige, Trento, Italy

12.10 – 12.30
Effects of ionic strength and sulfate upon thermal aggregation of grape chitinases and thaumatin-like proteins in model system
Matteo Marangon, The Australian Wine Research Institute, Glen Osmond, Australia

12.30 - 12.50
Progress in precision viticulture for the assessment of grape anthocyanins at harvest in australian vineyards
Marine Le Moigne, Centre Universitaire Orsay, Orsay Cedex, France

13.00 - 14.00
Lunch

SESSION II
BERRY DEVELOPMENT AND BIOSYNTHESIS OF GRAPE SECONDARY METABOLITES
Chairman: Serge Delrot

14.00 - 14.40
Keynote
REGULATION OF FLAVONOID BIOSYNTHESIS DURING GRAPE BERRY DEVELOPMENT
Jochen Bogs, University of Heidelberg, Heidelberg, Germany

14.40 - 15.00
Grapevine anthocyanin transport involves in vivo vesicle-mediated trafficking and the contribution of anthomate transporters and GST
Agnes Ageorges, INRA - UMR SPO, Montpellier, France

15.00 - 15.20
Cellular localization of leucoanthocyanidin reductase and anthocyanidin reductase in grape skins of vitis vinifera L. cv. Cabernet-Sauvignon during development
Soizic Lacampagne, Institut des Sciences de la Vigne et du Vin, Villenave d'Ornon, France

15.20 - 15.40
Cloning and characterization of putative anthocyanin acyltransferases from grape and petunia
S. Provenzano, University of Torino, Torino, Italy

15.40 - 16.10
Coffee break and poster viewing

16.10 - 16.30
New mybs through the glass: a deeper look into the regulatory network of flavonoid synthesis in wine grapes
J. Tomas Matus, Center for Research in Agricultural Genomics, Barcelona, Spain

16.30 - 16.50
Study of genetic determinants of flavonol content in grape berry skin
Giulia Malacarne, Fondazione Edmund Mach, San Michele All'Adige,Trento, Italy

16.50 - 17.10
Are VvMYB5A and VvMYB5B regulators of grape flavonoid pathway?
Giambattista Tornielli, University of Verona, Verona, Italy

17.10 - 17.30
Investigation of VvDXS function and its effects on Muscat flavor levels
Juri Battilana, IASMA Research and Innnovation Centre, San Michele All'Adige,Trento, Italy

17.30 - 17.50
Microclimate influence on gene expression and metabolite accumulation in grape berries
Christian Kappel, University of Bordeaux, Bordeaux, France

18.00 - 20.00
Welcome cocktail

Thursday, June 17

SESSION III
MOLECULAR EFFECTS OF STRESS AND VINEYARD MANAGEMENT
Chairman: Vittorino Novello

9.00 - 9.40
Keynote
POSTHARVEST ELICITORS AND METABOLIC CHANGES IN WINE GRAPE BERRIES
Piero Tonutti, Scuola Superiore Sant’Anna, Pisa, Italy

9.40 - 10.00
In vivo cellular localisation of stilbene fluorescence induced by Plasmopara viticola in grapevine leaves
Sebastien Bellow, Université Paris-Sud 11, Orsay, France

10.00 - 10.30
Biological role of resveratrol in plants
Philippe Jeandet, University of Reims, Reims, France

10.30 - 10.50
Effect of Botrytis Cinerea and Esca on berries and wines phenolic composition
Isabelle Ky, Institut des Sciences de la Vigne et du Vin, Villenave d'Ornon, France

10.50 - 11.10
Study of the early synthesis of resveratrol and its localization in grapevine cell cultures
David Donnez, UFR Reims, URVVC-SE, Reims, France

11.10 - 11.30
Coffee break and poster viewing

11.30 - 11.50
Relationship between physical and mechanical berries properties and phenol extractability of different varieties of Vitis Vinifera L. grapes
René Siret, Ecole Supérieure d’Agriculture, Angers Cedex, France

11.50 - 12.10
Effects of cluster thinning on berry ripening in cv. Sangiovese: agronomical, biochemical and molecular approach
Chiara Pastore, University of Bologna, Bologna, Italy

12.10 - 12.30
Evaluation of winegrape anthocyanins in the vineyard using a portable fluorimetric sensor: seasonal and water regime effects
Giovanni Agati, IFAC-CNR, Sesto F.no, Firenze, Italy

12.30 - 13.30
Lunch

13.30 - 23.00
Visit to the Castle of Cisterna d'Asti, to the Tenimenti di Fontanafredda Winery
(Serralunga d'Alba) and social dinner at the Villa Contessa Rosa Resturant

Friday, June 18

SESSION IV
RELATIONS BETWEEN SECONDARY METABOLITES AND WINE SENSORY PROPERTIES
Chiarman: Vincenzo Gerbi

9.00 - 9.40
Keynote
CURRENT STATUS IN POLYSACCHARIDE AND OLIGOSACCHARIDE COMPOSITION OF WINES
Thierry Doco, INRA Montpellier, France

9.40 - 10.00
Toward a model of grape proanthocyanidin extraction during vinification
Keren Bindon, Australian Wine Research Institute, Adelaide, Australia

10.00 - 10.20
Ultra high pressure liquid chromatography for stilbenes separation and their determination in Burgundy red wines
Lemia Boutegrabet, Burgundy University, Dijon, France

10.20 - 10.40
Stilbene-enriched red quality wine upon UVC postharvest treatment
Emma Cantos-Villar, IFAPA, Jerez de la Frontera, Cadiz, Spain

10.40 - 11.00
Evolution of biogenic amines content in wine during sample conservation - method optimisation for analysis of biogenic amines
Benoit Bach, Inter Rhone, Orange, Cadiz, France

11.10 - 11.30
Coffee break and poster viewing

11.30 -11.50
Effect of winemaking techniques on the polysaccharide and oligosaccharide composition of Cabernet Sauvignon, Syrah and Monastrell red wines
Rafael Apolinar-Valiente, University of Murcia, Murcia, Spain

11.50 -12.10
Phenolic composition of bordeaux grapes 2009 vintage: comparison with 2006, 2007 and 2008 vintages
Bénédicte Lorrain, Institute des sciences de la Vigne et du Vin, Villenave d'Ornon, France

12.10 - 12.30
PH and ionic strength impact on protein stability in model white wines at room temperature
Marie Dufrechou, INRA, Montpellier, France

12.30 - 12.50
Changes in wine secondary metabolites composition by the timing of inoculation with lactic acid bacteria: impact on wine aroma
Guillaume Antalick, ISVV Faculty D'Oenologie Bordeaux, Villenave d'Ornon, France

13.00 - 14.00
Lunch

SESSION V
IMPACT OF MICROBIOLOGICAL AND ENOLOGICAL TECHNIQUES ON WINE MACROMOLECULES
Chairperson: Elizabeth Waters

14.00 - 14.40
Keynote
CHEMICAL SYSTEMS BEHIND WINE AROMA PERCEPTION: OVERVIEW, GENESIS AND EVOLUTION
Ana Escudero, University of Zaragoza, Zaragoza, Spain

14.40 - 15.00
Chemical and sensory evaluation of Bordeaux wines (Cabernet-Sauvignon and Merlot) and correlation with wine age
Kleopatra Chira, Institute des sciences de la Vigne et du Vin, Villenave d'Ornon, France

15.00 -15.20
Different oxygen and sulphur dioxide concentrations in Sauvignon blanc must: effect on the composition of the must and wine
Wessel Du Toit, University of Stellenbosch, Cape Town, South Africa

15.20 - 15.40
Identification, quantification and organoleptic impact of 3-METHYL-2,
4-NONANEDIONE, a new volatile compound associated with the oxidation of red wines
Alexandre Pons, Institute des sciences de la Vigne et du Vin, Villenave d'Ornon, France

15.40 - 16.10
Modulation of wine aroma composition and sensory properties through management of fermentation and post-fermentation enological variables
Maurizio Ugliano, Australian Wine Research Institute, Glen Osmond SA, Australia

16.10 - 16.30
Coffee break and poster viewing

16.30 - 16.50
Brettanomyces Bruxellensis and off-odours: genetic and proteomic approaches to unravel the molecular mechanism of ethyl phenols production
Luca Cocolin, University of Torino, Torino, Italy

16.50 - 17.10
Sensory evaluation of Sauvignon blanc grapes by a trained panel
Erna Witbooi, University of Stellenbosch, Cape Town, South Africa

17.10 - 17.30
Extraction and identification of phenolic and volatile compounds in cork closures
Maria A. Silva, Institut des Sciences de la Vigne et du Vin, Villenave d'Ornon, France

17.30 - 18.00
Final discussion