PROTEOMIC SCHOOL
CNR-ISPA c/o Bioindustry Park, Colleretto Giacosa (TO)

The school will provide theoretical and practical basis on techniques for protein separations, analysis and identification.

Advanced practical module on:
BIDIMENSIONAL SEPARATION OF PROTEINS

April 19-21, 2006

October 25-27, 2006
*** lista d'attesa ***

with the patronage of

Gruppo Interdivisionale Chimica del Proteoma


- Sample preparation
- 2 D gel Electrophoresis
- 2 D LC
- Protein detection
- Image analysis
- Technical challenges

Upon previous presentation of specific samples or protein extract, participants will be allowed to use them during the course and to present papers related to the topics.
Maximum number of participants: 5

1st day

9.00
participants registration/ registrazione partecipanti

9.30
course presentation/ Presentazione del corso (Conti)

10.00
2DE tecniques/ tecniche di estrazione e di separazione delle proteine (A. Conti)

10.30 – 10.45
coffee break

10.45
Principles of 2D Electrophoresis/ Principi della 2-D Elettroforesi (Conti)

11.30
Principles of 2-D LC/ Principi della 2-D LC*(Reuter)
*This talk will be in English

12.30
Lunch

14.00
Sample preparation theory/ Teoria sulla preparazione del campione (Barello)

15.00 – 17.30
Practical lab work/ Prove pratiche di estrazione e preparazione della prima dimensione (Baro, Cereti, Dellavalle, Reuter)

2nd day

9.00
Theory on tecniques for the identification of proteins separated by 2D methods/ Teoria sulle tecniche di identificazione delle proteine separate mediante metodi 2D (Giuffrida)

10.45 – 11.00
coffee break

11.00
Introduction to the use of image analisys programs/ Introduzione all'uso di programmi di analisi d’immagine (Baro)

12.30
Lunch

14.00 – 17.30
Practical lab work/ Preparazione della seconda dimensione, colorazione dei gel (Baro, Cereti, Dellavalle, Reuter)

3rd day

9.30
Gel acquisition/ Acquisizione dei gel (Baro, Dellavalle)

10.00 
2D Gel practical session/ Prove pratiche per seconda dimensione (Baro, Dellavalle)

10.45 – 11.00
coffee break

11.00
ECM test and discussion/ Test ECM, discussione e commenti

12.00
End of course/ Fine del corso

Beginning: 19 April e 25 October 9.30 am
Conclusion: 21 April e 27 October 12.00 am

Deadline for 2D Gel Electrophoresis applications:
19 March and 30 September, 2006

General information
Scientific Coordinator: Dott. Amedeo Conti, ISPA-CNR Torino

The course will be held in Italian if all participants are Italian speakers otherwise the lessons will be in English.

Course Location
CNR-ISPA c/o Bioindustry Park, Via Ribes, 5 Colleretto Giacosa, To, (I)
Registration fee
for each module is € 650 (VAT 20% included) net of any bank charge.
Registration fee includes:
- Transfer by bus from the hotels to Bioindustry Park and back – for all course duration
- Conference kit
- Attendance certificate
- Coffee breaks, meals

2D Gel Electrophoresis is going to be acknoledged by the Italian Ministry of Health.
15 Credits will be given to Italian graduates in Medicine, Biology, Pharmacy and Chemistry (146-233296), Lab Technicians (146-233297) working in appropriate sectors and filling relative ECM documents.
For updates please visit:  http://ecm.sanita.it

Organising Secretariat
Dott.ssa Elisabetta Lombardini
Fondazione per le Biotecnologie
Viale Settimio Severo, 63 - 10133 TORINO (I)
Phone +39 011 6600187 - Fax +39 011 6600708
e-mail: elisabetta.lombardini@fobiotech.org
Accommodation
List of useful hotels is available contacting the organizing secretariat.

[ go to the registration form ]